Sunday, August 14, 2011
Can you help me find online sources on isolation of hemin from blood? I need chemical equations to it up.?
The procedure is as follows: The objective of this experiment is to successfully isolate hemin from pig’s blood by precipitating and filtering contaminant proteins and by decomposing hemoglobin to hemin by addition of glacial acetic acid saturated by strontium chloride, in acetone solvent. The crude hemin is then concentrated and crystallized, before being centrifuged. By washing with acetic acid and water, then with ethanol and ether, then recrystallized with pyridine, and purified with chloroform, the hemin is then removed of other contaminants. It is then added again with strontium chloride in glacial acetic aced, then again centrifuged and recrystallized.
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